Don James Konovsky
Preceptor: James Guest, MD, PhD
Institution: University of Miami
Research Summary/Abstract: The aim of this research project is to address whether or not quantum dots (Qdots) can be stably incorporated into glial cells and can be uniquely identifiable within such cells when viewed using transmission electron microscopy. The intracellular appearance (including the presence of membrane incorporation of Qdots) will be studied at 2500 - 12000x, and the labeling efficiency, particles per cell, and stability within target cells will be quantified. If such incorporation occurs and is identifiable, then the labeled cells may serve as very useful reagents for transplantation studies. This will aid in improving upon the current techniques that are used in assessing the integration of transplanted cells into injured CNS parenchyma. |